tumour cells u87  (SAS institute)

Journal: Scientific reports

Article Title: Repeated 5-aminolevulinic acid mediated sonodynamic therapy using magnetic resonance guided focused ultrasound in rat brain tumour models.

doi: 10.1038/s41598-025-85314-6

Figure Lengend Snippet: Fig. 4. (A) We further investigate the efficiency of SDT in the human glioblastoma U87 rat model. The animal received three weekly treatments beginning from day 7. (B) The representative T1 contrast-enhanced MR images of control, FUS alone, 5-ALA alone, and SDT treatment. The first row in the SDT group demonstrated the long-term tumour-free survivor. The tumour disappeared after three treatments. However, SDT had a minimal impact on some animals, such as the second row in the SDT group. (C) The H&E stains stood for the animals from (B), respectively. (D) A significant difference was found in the third week between the control group and the SDT group. (E) The log-rank test showed prolonged survival in the SDT group, p = 0.01. (F) The body weight in all groups did not vary during three-week treatments.

Article Snippet: Rodent 9L gliosarcoma and human U87 MG glioma brain tumour cells Rodent 9L gliosarcoma brain tumour cells (ATCC® CRL-2200TM) and human U87 MG glioblastoma (ATCC® HTB14TM) purchased from ATCC were cultured respectively in DMEM (Dulbecco’s modified Eagle’s medium) and EMEM (Eagle’s Minimum Essential Medium) and supplemented with 10% heat-inactivated fetal bovine serum (FBS) in 75 cm2 flasks in a 5% CO2-containing incubator at 37 °C.

Techniques: Control

Journal: Cancers

Article Title: A Novel Therapeutic Strategy for the Treatment of Glioma, Combining Chemical and Molecular Targeting of Hsp90α

doi: 10.3390/cancers3044228

Figure Lengend Snippet: Hsp90α expression in U87-MG post 17-AAG and sihsp90α treatment. The Hsp90α levels were deduced as the fraction of the observed Hsp90α levels in treated samples relative to the total Hsp90α levels in the untreated sample using immunofluorescence after 48 h and 72 h. Hsp90α expression analysis using immunofluorescence was performed by counting 150 cells. sihsp90α represent cells treated with hsp90α specific siRNA. sihsp90α + 17-AAG represent concurrent assays where the cells were treated with 17-AAG and sihsp90α for either 48 h or 72 h. This data is typical of three independent experiments. * p ≤ 0.05 and ** p ≤ 0.001 were considered statistically significant.

Article Snippet: The human brain tumour cell line U87-MG (glioblastoma multiforme) and human normal astrocyte cell line SVGp12 were purchased from European Collection of Cell Cultures (ECACC) and the American Type Culture Collection (ATCC), respectively.

Techniques: Expressing, Immunofluorescence, Control